Nuclear Factor-κB Plays a Positive Role in TNFα Expression in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)

Tumour necrosis factor-α (TNFα) is a multifarious mediator of lymphoid tissue growth and antimicrobial defence mechanisms, it acts as pro-inflammatory regulator. The function TNFα in parasite infection the underlying mechanism through which nuclear factor-κB (NF-κB) regulates remain largely unclear teleosts. In present study, ( ToTNF α) from golden pompano Trachinotus ovatus ) was identified, its sequence features expression levels were determined. genomic DNA composed 1,130 bp, consists four exons three introns, encodes 341 amino acid polypeptides. putative protein shares 34.7%–61.9% identity with fish possesses TNF family signature two conserved cysteine residues. Moreover, expressions ToNF- κ B α are constitutively expressed all examined tissues, higher observed immune relevant tissues. Both transcription increased local sites (skin gill) system tissues (liver, spleen head kidney) after Cryptocaryon irritans stimulation. addition, to investigate whether ToNF-κB regulator α, promoter analysis performed. region -970 +79 bp known core by different truncated mutants α. Subsequently, activity -p2 dramatically reduced targeted mutation M6-binding site. Additionally, an electrophoretic mobile shift assay (EMSA) verified that interacted site control conclusion, study provides positive regulation NF-κB contributes better understanding transcriptional fish.